摘要
AIM: To investigate the inhibitory effect of a Chinese herb medicine Astragali radix (AR) on growth of different cancer cell lines.METHODS: To observe the in vitro effects of AR on tumor cell proliferation by trypan blue exclusion, MTS method and tritium thymidine incorporation assay. Apoptosis was detected by DNA ladder method.RESULTS: The inhibition rates of AR on the cell respiration of AGS, KATOⅢ, HT29, MDA231, MEL7 and MEL14 were 68.25%, 62.36%, 22.8%, 27.69%, 2.85% and 5.14%respectively at the concentration of 100 ug/ml; it inhibited AGS DNA synthesis by 87.33% at the concentration of 50ug/ml. The inhibitory effect on AGS was time-and dosedependent. AR did not induce apoptosis in AGS cells.CONCLUSION: AR specifically inhibits gastric cancer cells growth in vitro and the mechanism is mainly cytostatic but not cytotoxic or inducing apoptosis.
AIM:To investigate the inhibitory effect of a Chinese herb medicine Astragali radix(AR)on growth of different cancer cell lines. METHODS:To observe the in vitro effects of AR on tumor cell proliferation by trypan blue exclusion,MTS method and tritium thymidine incorporation assay.Apoptosis was detected by DNA ladder method. RESULTS:The inhibition rates of AR on the cell respiration of AGS,KATOIII,HT29,MDA231,MEL7 and MEL14 were 68.25 %,62.36 %,22.8 %,27.69 %,2.85%and 5.14 % respectively at the concentration of 100 ug/ml;it inhibited AGS DNA synthesis by 87.33%at the concentration of 50 ug/ml.The inhibitory effect on AGS was time-and dose- dependent.AR did not induce apoptosis in AGS cells. CONCLUSION:AR specifically inhibits gastric cancer cells growth in vitro and the mechanism is mainly cytostatic but not cytotoxic or inducing apoptosis.