摘要
AIM: To construct a recombinant strain which highly expresses catalase of Helicobacter pylori(H.pylori) and assay the activity of H. pylori catalase.METHODS: The catalase DNA was amplified from H. pylori chromosomal DNA with PCR techniques and inserted into the prokaryotie expression vector pET-22b (+), and then was transformed into the BL21 (DE3) E. coli strain which expressed catalase recombinant protein. The activity of H.pylori catalase was assayed by the Beers & Sizers.RESULTS: DNA sequence analysis showed that the sequence of catalase DNA was the same as GenBank's research. The catalase recombinant protein amounted to 24.4 % of the total bacterial protein after induced with IPTG for 3 hours at 37 ℃ and the activity of H. pylori catalase was high in the BL21 (DE3) E. coli strain.CONCLUSION: A clone expressing high activity H. pylori catalase is obtained, laying a good foundation for further studies.
AIM:To construct a recombinant strain which highly expresses catalase of Helicobacterpylori(H.pylori) and assay the activity of H.pylori catalase. METHODS:The catalase DNA was amplified from H.pylori chromosomal DNA with PCR techniques and inserted into the prokaryotie expression vector pET-22b (+),and then was transformed into the BL21 (DE3) E.coli strain which expressed catalase recombinant protein.The activity of H. pylori catalase was assayed by the Beers & Sizers. RESULTS:DNA sequence analysis showed that the sequence of catalase DNA was the same as GenBank's research.The catalase recombinant protein amounted to 24.4% of the total bacterial protein after induced with IPTG for 3 hours at 37℃ and the activity of H.pylori catalase was high in the BL21 (DE3) E.coli strain. CONCLUSION:A clone expressing high activity H.pylori cataiase is obtained,laying a good foundation for further studies.
基金
the National Natural Science Foundation of China, No.30270078
