Leptin receptor expression in the basolateral nucleus of amygdala of conditioned taste aversion rats

AIM: To determine whether serum leptin level and the leptin receptor (OB-R) expression in the basolateral amygdala (BLA)change following conditioned taste aversion (CTA) formation.METHODS: The serum leptin concentration was measured by rat leptin RIA kit, long and short forms of leptin receptor (OB-Rb and OB-Ra) mRNA in the brain sections were examined by in situ hybridization (ISH) and the expression of OB-R was assessed by immunohistochemistry ABC method with a highly specific goat anti-OB-R antibody.RESULTS: The level of serum leptin didn't show significant difference between CTA and control group. Comparing with the control group, the CTA group had an increase on count of OB-R immunohistochemistry positive-stained cells in the BLA (127±12 vs 48±9 per 1 mm2). The OB-Rb mRNA expression level enhanced by 11.9 % in the BLA, while OBRa mRNA level increased by 7.4 % on the choroid plexus in CTA group. So BLA was supposed to be a region where interactions between gustatory and vagal signals take place.CONCLUSION: BLA is one of the sites, which are responsible for CTA formation in the brain. Leptin and OB-R maybe involved in neuronal communication for CTA. So leptin and its receptors probably take part in CTA and integration of autonomic and extroceptive information.

AIM:To determine whether serum leptin level and the leptin receptor (OB-R) expression in the basolateral amygdala (BLA) change following conditioned taste aversion (ERA) formation. METHODS:The serum leptin concentration was measured by rat leptin RIA kit,long and short forms of leptin receptor (OB-Rb and OB-Ra) mRNA in the brain sections were examined by in situ hybridization (ISH) and the expression of OB-R was assessed by immunohistochemistry ABC method with a highly specific goat anti-OB-R antibody. RESULTS:The level of serum leptin didn't show significant difference between CTA and control group.Comparing with the control group,the CTA group had an increase on count of OB-R immunohistochemistry positive-stained cells in the BLA (127±12 vs 48±9 per 1 mm^2).The OB-Rb mRNA expression level enhanced by 11.9% in the BLA,while OB- Ra mRNA level increased by 7.4% on the choroid plexus in CTA group.So BLA was supposed to be a region where interactions between gustatory and vagal signals take place. CONCLUSION:BLA is one of the sites,which are responsible for CTA formation in the brain.Leptin and OB-R maybe involved in neuronal communication for CTA.So leptin and its receptors probably take part in CTA and integration of autonomic and extroceptive information.