肝硬化患者血小板胞内Ca^(2+)浓度、CD_(62)P、CD_(63)及血浆CD_(62)P的检测

Detection of platelet Ca^(2+i), CD_(62)P, CD_(63) and plasma CD_(62)P in cirrhosis patients

l摘fi 目的 探讨肝硬化患者与血小板a一颗粒膜蛋白(CD。。P)、血小板糖蛋白(CD。。)、血浆CD。f(SCD。I)水平及血小板[Ca’‘＊的关系。方法 利用流式细胞术检测血小板 CD。。P、CD。。,酶联免疫吸附法测定SCD。f和荧光分光光度法测定血小板[Ca‘”h。结果 肝硬化患者血小板[Ca’”]i、CD。。P、CD。。、SCD。;P分别为(103.l士 22.2)nmol/L、(47.6上 20.0)％、(47.1士 24.6)％和(67.6士 37.6)no/L,对照组分别为(57.6 t13.l)nmol/L、(3.h 0,7)％、(2.510.7)％和(24.016.5)ug/L,两组比较差异有显著性,t值为6.148—19.067,P<0.05;上消化道出血组分别为(120.3 i 18.8)urn。l/L、(64.9 i 14.7)％、(7.9 i 14二5)％和(103.6士 14.9)ug/L,无出血组分别为(91.l士 14.3)nmol/L、(3.6士 11.9)％、(30.2士 14.4)％和(40.8 i 24.0)u0.05)。结论 肝硬化患者血小板高度活化,检测血小板CD62P、CD63、SCD62P对判断肝硬化的严重程度有一定参考价值。

Objective To study on the relationship between platelet Ca2+i, CD62P, CD63, serum CD62P (SCD62P) and cirrhosis patients. Methods Platelet CD62P, CD63 were determined with flow cytometry, SCD63P with ELISA, and Ca2+i in platelet was determined with fluorophotometry. Results Platelet Ca2+i, CD62P, CD63, and SCD62P levels in cirrhosis patients were (103.1 ± 22.2) nmol/L, (47.6 ± 20.0) %, (47.1 ± 24.6) %, and (67.6 ± 37.6)μg/L, and in controls were (57.6 ± 13.1) nmol/L, (3.1 ± 0.7) %, (2.5 ± 0.7) %, and (24.0 ± 6.5)μg/L, respectively. The levels in the former were higher than those in the latter (t ≥ 6.148, P < 0.05). The above levels in upper gastrointestinal haemorrhage group were much higher than those in the non- haemorrhage group (120.3 nmol/L + 18.8 nmol/L vs 91.1 nmol/L + 14.3 nmol/L, 64.9% ± 14.7% vs 34.6% ± 11.9%, 70.9% ± 14.5% vs 30.2% ± 14.4%, and 103.6μg/L ±14.9μg/L vs 40.8μg/L ± 24.0μg/L, respectively, t ≥ 5.380, P < 0.05). But the numbers of platelet between the two groups were no obvious difference. Conclusions Platelet in the cirrhosis patients is greatly active, and the detection of platelet CD62P, CD63, SCD62P has a certain value in judging the degree of cirrhosis.