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多种方法研究乙型肝炎病毒多聚酶变异 被引量:5

Study the mutations in polymerase gene of hepatitis B virus by three methods.
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摘要 以DNA测序结果为标准,采用寡核苷酸芯片技术和基于荧光定量聚合酶链反应(FQ-PCR)的UniArray技术分析24例使用拉米夫定治疗12个月以上的慢性乙型肝炎(CHB)患者YMDD变异的情况,检测HBV DNA和丙氨酸氨基转移酶(ALT)水平,以探讨上述两种方法检测YMDD突变的价值及拉米夫定治疗乙型肝炎过程中出现的耐药状况.
出处 《中华肝脏病杂志》 CAS CSCD 2003年第7期442-443,共2页 Chinese Journal of Hepatology
关键词 乙型肝炎病毒 多聚酶变异 多聚合酶链反应 YMDD变异 治疗 Hepatitis B virus Mutation Polymerase chain reaction
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同被引文献33

  • 1王磊,闫杰,张照华,王晶波,杜以真,李晓迎,王耀宗.拉米夫定治疗慢性乙型肝炎患者YMDD变异及影响因素的研究[J].中华肝脏病杂志,2004,12(10):585-588. 被引量:27
  • 2Rong-Sheng Wang,Hua Zhang,Yu-Fen Zhu,Bei Han,Zhi-Jun Yang.Detection of YMDD.mutants using universal template real-time PCR[J].World Journal of Gastroenterology,2006,12(8):1308-1311. 被引量:17
  • 3汪小娟,刘香萍,罗尧都,叶国强,谢春英.3种检测乙型肝炎病毒YMDD变异方法比较及结果分析[J].中国感染控制杂志,2006,5(2):102-104. 被引量:4
  • 4Funaki NO, Tanaka J, Itami A, Kasamatsu T, Ohshio G,Onodera H, Monden K, Okino T, Imamura M. Detection of colorectal carcinoma cells in circulating peripheral blood by reverse transcription-polymerase chain reaction targeting cytokeratirr-20 mRNA. Life Sci 1997:60:643-652.
  • 5Nishida S, Kitamura K, Ichikawa D, Koike H, Tani N, Yamagishi H. Molecular detection of disseminated cancer cells in the peripheral blood of patients with gastric cancer. Anticancer Res 2000;20:2155-2159.
  • 6Chausovsky G, Luchansky M, Figer A, Shapira J, Gottfried M, Novis B, Bogelman G, Zemer R, Zimichman S, Klein A. Expression of cytokeratin 20 in the blood of patients with dissminated carcinoma of the pancreas, colon, stomach, and lung. Cancer 1999;86:2398-2405.
  • 7Ikeguchi M, Ohro S, Maeda Y, Fukuda K, Yamaguchi K, Shirai H, Kondo A, Tsujitani S, Kaibara N. Detection of cancer cells in the peripheral blood of gastric cancer patients. Int J Mol Med 2003;11:217-221.
  • 8Majima T, Ichikura T, Takayama E, Chochi K, Mochizuki H. Detecting circulating cancer cells using reverse transcriptase polymerase chain reaction for cytokeratin mRNA in peripheral blood from patients with gastric cancer. Jpn J Clin Oncol 2000;30:499-503.
  • 9Shin JH, Chung J, Kim HO, Kim YH, Hur YM, Rhim JH, Chung HK, Park SC, Park JG, Yang HK. Detection of cancer cells in peripheral blood of stomach cancer patients using RT-PCR amplification of tumour specific mRNAs. Aliment Pharmacol Ther 2002;16:137-144.
  • 10Kodera Y, Nakanishi H, Ito S, Yamamura Y, Kanemitsu Y, Shimizu Y, Hirai T, Yasui K, Kato T, Tatematsu M. Quantitiative detection of disseminated free cancer cells in peritoneal washes with real-time reverse transcriptase-poly merase chain reaction :a sensitive predictor of outcome for patients with gastric carcinoma. Ann Surg 2002;235:499-506.

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