宫颈癌组织TGF-β_1、TGF-βR_1和p27kipl表达及临床意义

Clinical Significance of TGF-β_1,TGF-βR_1 and p27kipl Expression in Cervical Carcinoma

目的 :研究p2 7Kip在宫颈癌中的表达与TGF β1 配体和TGF β1 受体表达之间的关系。方法 :选择 6 3例宫颈标本 ,包括 2 8例良性宫颈内膜腺体 ,8例宫颈原位腺癌和 2 7例宫颈腺癌 ,采用S P方法检测多克隆抗体TGF β1 、TGF β1 受体和单克隆抗体p2 7Kip表达情况。结果 :正常的子宫颈内膜标本中 ,TGF β1 显示出弱的胞质染色 ,而p2 7Kip显示胞核强阳性 ,与正常宫颈组织相比 ,随着肿瘤恶性程度的增加 ,TGF β1 配体和TGF β1 受体胞质染色随之增强。在腺癌中TGF β1 的表达比原位宫颈癌中表达变弱 ,但比正常的子宫内膜仍有显著性增强 ,p2 7Kip蛋白的表达随着肿瘤恶性程度增加而下降 ,而正常的子宫内膜则显示出最强的p2 7Kip蛋白的表达。结论 :子宫内膜的肿瘤转化可能与TGF β1 和p2

Objective To investigate the relationship between the expression of the TGF β 1 ligands and TGF β 1 receptors with the expression of p27kipl in cervical cancer.Methods Sixty three formalin fixed and paraffin embedded human cervical specimens,including 28 with benign endocervical glands,8 with cevical adenocarcinoma in situ and 27 with cervical adenocarcinomas were exanmined by using the immunochesitry method with polyclonal antibodies that react specifically to TGF β 1,TGF βR 1 and p27kipl.Results In normal endocervix,the TGF β 1 showed weak cytoplasmic staining,while p27kipl1 showed strong nuclear staing.High expression of TGF β 1 ligand and leceptor was observed as tumor upgrading,expression of TGF β 1 was lower in adenocarcinoma than that in adenocarcinoma in situ,but still significantly higher than that in normal endocervix.p27kipl protein expression decreased,while normal endocervix showed the highest level of p27kipl protein expression.Conclusions Neoplastic transformation of the cervix may be related to dysregulation of TGF β 1 and p27kipl.