外阴鳞癌组织MDM2 mRNA和p53蛋白表达及其意义

Expression and Significance of MDM2 mRNA and p53 Protein in Squamous Cell Carcinoma of the Vulva

目的 :探讨外阴鳞癌组织中MDM2mRNA和p5 3蛋白的表达及在外阴鳞癌发生、发展中的作用。方法 :采用RT PCR法检测 31例外阴鳞癌组织中MDM2mRNA表达情况 ,利用免疫组化S P法检测p5 3蛋白的表达 ,并与 31例癌旁正常外阴组织进行对照。结果 :在外阴鳞癌组织中MDM2mRNA表达率为 2 2 6 % ,明显高于对照组 ,P <0 0 5。MDM 2表达与组织分化程度无关 ,P >0 0 5。外阴鳞癌组织中p5 3蛋白表达阳性率为 5 8 1 % ,明显高于对照组。突变型p5 3在低、中、高分化外阴鳞癌组中的表达率分别为 87 5 %、6 4 3%及 2 2 2 % ,其表达率随着分化程度的降低而升高 ,低分化组中的表达率明显高于高分化组 ,P <0 0 5。MDM2基因扩增与p5 3蛋白表达呈正相关 ,P <0 0 5。结论 :MDM2基因过表达是外阴鳞癌发生、发展过程中较常见的分子事件 ;p5 3基因突变在外阴鳞癌发生、发展中起重要作用。检测p5

Objective To investigate the significance of expressions of MDM2 mRNA and p53 protein in squamous cell carcinoma of the vulva (VSCC).Methods Thirty one cases of VSCC and vulvar epithelia adjacent to the tumour were detected for the expressions of MDM2 mRNA and p53 protein with RT PCR and immunohistochemistry S P methods respectively.Results The expressions of MDM2 mRNA and p53 protein in VSCC were 22 6%(7/31) and 58 1%(18/31) respectively.The expression of p53 in lower grade group was much higher than that in higher grade group, P <0 05.There was the correlation between the expression of MDM2 mRNA and p53 protein, P <0 05.Conclusion In VSCC,the proliferation of MDM2 gene is a usual event and the detection of p53 protein may be helpful to assess the prognosis of VSCC.