珍珠梅提取物对二乙基亚硝胺所致大鼠肝脏癌前病变的抑制作用及抗氧化活力的影响(英文)

Inhibitory Effect of Sorbaria Sorbifolia on DEN-induced Precancerous Hepatic Foci and Its Antioxidative Activities in Rats

目的 :研究珍珠梅对二乙基亚硝胺致大鼠肝脏癌前病变灶及抗氧化活力的抑制作用。方法 :75只大鼠随机分为A、B、C 3组 :对照组、模型组和治疗组 ,每组 2 5只。按略改良的Solt Farber氏方法制作大鼠肝脏癌前病变模型 ,用珍珠梅提取物饲养大鼠 6周后处死 ,通过免疫组织化学检测癌前病变组织谷胱甘肽S -转移酶 (GST P)和p5 3蛋白的表达 ,放射免疫法检测血清肿瘤坏死因子 (TNF α)的含量 ,比色分析法检测血清、肝线粒体超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH Px)的活性及丙二醛(MDA)的含量。结果 :B和C组GST P的表达率为 86 7%和 2 8 6 % ,p5 3的表达率为 5 6 %和 36 % ,差异均有显著意义 ,P <0 0 5 ;B组血清TNF α的含量与C组比较 ,差异有显著意义 ,P <0 0 5 ;B组血清、肝线粒体SOD、GSH Px活性及MDA含量与C组比较 ,差异均有显著意义 ,P <0 0 5。结论 :珍珠梅提取物对DEN所致大鼠癌前病变灶有抑制作用 ,可诱发TNF α的生成 ,并有抗氧化作用。

Objective To investigate the effect of Sorbaria sorbifolia on diethylnitrosamine (DEN) induced precancerous hepatic foci and its antioxidative activities in rats.Methods According to the standard method,75 rats were randomly divided into 3 groups:Group A (control),Group B (model),Group C (treatment),and 25 rats in each group.Hepatic precancerous lesions in rats were induced by the modified Solt and Farber protocol and the rats were sacrificed after feeding Sorbaria sorbifolia extract for 6 weeks.The expressions of glutathine S-transferase (GST-P),p53 protein were investigated by immunohistochemistry techniques.TNF-α was measured by the radioimmunoassay and antioxidative activities of SOD,GSH-Px and the content of MDA were investigated by the colorimetric method in rat serum and mitochondria of liver cells.Results GST-P stainting was 86.7% positive in Group B and 28.6% positive in Group C;p53 protein was 53.3% in Group B and 38.1% in Group C positive respectively.For the content of serum TNF-α,the activities of SOD and GSH-Px,and the content of MDA in the serum and liver cells mitochondria,there were significant differences between Group B and Group C.Conclusions Sorbaria sorbifolia extract has inhibitory effect on DEN-induced precancerous hepatic foci in rats and induces TNF-α production in rats.The antioxidative action is exhibited by the administration of Sorbaria sorbifolia extract in the early stage of chemical hepatocarcinogenesis in rat livers.