子宫内膜癌P_(53)基因突变与侵袭转移的关系及意义

Study of the P_()53 Gene Mutation and Its Relation to the Invasion and Metastasis in Endometrial Carcinoma

目的 研究子宫内膜癌组织中P53基因突变及其蛋白表达与癌组织侵袭、转移间的关系。方法 应用免疫组织化学技术 ,对 63例子宫内膜癌组织进行P53基因突变及蛋白表达的原位检测 ,并在不同病变组间进行对比分析。结果 子宫内膜癌组织中P53基因突变与癌组织肌层浸润深度及淋巴结转移密切相关 ,且随肌层浸润深度的增加和淋巴结转移的出现 ,P53基因的突变率及蛋白表达强度增加。深肌层浸润组P53基因突变率为 71.4 3%、蛋白表达强阳性率为 5 2 .38% ,均高于浅肌层浸润 (40 % ,P <0 .0 5 ;14.2 9% ,P <0 .0 1)和无肌层浸润组 (14.2 9 % ,P <0 .0 5 ;0 ,P <0 .0 5 )。有淋巴结转移组P53基因突变率为 93.33% ,蛋白表达强阳性率为 80 .0 0 % ,高于无淋巴结转移组 (33.33% ,P <0 .0 1;8.33% ,P <0 .0 1)。结论 子宫内膜癌组织中P53基因的突变 ,参与了其生物学行为的调控 。

Objective To study the P 53 gene mutation and protein expression in endometrial carcinoma and its relation to the invasion and metastasis of endometrial carcinoma.Methods Immuno-histochemical technique was used to measure the P 53gene mutation and protein expression in 63 cases with endometrial carcinoma in situ and the results were analyzed in different groups.Results The P 53 gene mutation was closely relevant to the invasion depth and lymph node metastasis,and with the increase in invasion depth and the advent of lympth node metastasis,the The P 53 gene mutation and protein expression increased.The P 53 gene mutation and the strongly positive expression rate of the protein were higher in endometrial carcinoma with deep layer invasion (71.43%,52.38%) than those of the shallow layer invasion ones (40.00%,14.29%) (χ 2= 5.19,P<0.05;χ 2=7.56,P<0.01) and no invasion ones(33.33%,8.33%) (χ 2=4.86,P<0.05;χ 2=4.04,P<0.05).They were also higher in endometrial carcinoma with lymph node metastasis (93.33%,80.00%) than others without lymph node metastasis(33.33%,8.33%) (χ 2=14.18,P<0.01;χ 2=27.31,P<0.01).Conclusion The P 53 gene mutation may play an important role in the invasion and metastasis of endometrial carcinoma.