汉族人芳香二烷基磷酸酯酶启动子-162A/G基因多态性与冠心病发病的关系

Relationship between PON1-162A/G gene polymorphism and CHD

目的 研究湖北地区汉族人芳香二烷基磷酸酯酶 (paraoxonasc ,PON1)启动子基因 -162位Ala -Gly基因多态性与冠心病 (CHD)发病的关系。方法 应用多聚酶链反应 -限制性片段长度多态性的分析方法 (PCR -RELP)检测湖北汉族地区CHD患者及正常对照组PON1基因启动子 -162位点的多态性 ,血脂用酶法测定。结果 PON1启动子 -162位点存在多态性 ,有AA ,AG ,GG 3种基因型。 12 8例正常人中AA基因型占 4 7% ,AG基因型占 2 0 3 % ,GG基因型占 75 0 % ,而CHD组与对照组 -162Ala-Gly 基因型分布总体构成比差异无显著性。对照组和CHD组PON基因G等位基因频率分别为 0 85 2和0 913 ,差异也无显著性 (P >0 0 5 )。结论 未发现PON1启动子基因 -162位Ala-Gly多态性与中国人CHD有关。

To study the relationship between PON1- 162A/G gene polymorphism and CHD of the Han nationality in Hubei.PCR - RELP was applied in detecting PON1- 162A/G gene polymorphism of CHD group and contrast group. Polymorphism existed in PON1- 162. There were 3 kinds of genotypes: AA, AG, GG. 4.7% of 128 normal people was AA genotype, 20.3% was AG genotype, and 75.o% was GG genotype. No significant difference was found in constituent ratio of 162Ala -Gly genotype distribution between CHD group and contrast group. Gene frequencies of contrast group and CHD group were 0.852 and 0.913 respectively. No significant difference was found (P> 0.05).[Conclusion]There is no relationship between PON1- 162Ala - Gly gene polymorphism and Chinese CHD.