胃肠MALT淋巴瘤API2-MALT1融合基因表达对肿瘤细胞增殖和凋亡相关蛋白的影响

The Expression of API2-MALT1 Fusion Gene and Its Influence on the Cellular Apoptosis and Proliferation in Gastrointestinal MALT Lymphoma

目的 探讨胃肠道粘膜相关淋巴组织来源的结外边缘区 B细胞 (MAL T)淋巴瘤中 API2 - MAL T1融合基因表达对凋亡相关蛋白 API2、Caspase3表达及肿瘤细胞增殖活性的影响。方法 用 RT- PCR和巢式 PCR结合 ,检测肿瘤组织中 API2 - MAL T1融合基因的表达 ;根据有否该融合基因 m RNA表达将 33例病例分组 ,用免疫组化染色检查肿瘤组织中 Ki6 7、API2和 Caspase3蛋白的表达。结果 16例胃肠 MAL T淋巴瘤检出 API2 -MAL T1m RNA的表达 ,检出率 4 8.4 %。融合基因表达组 API2平均面积 (2 1177.30± 6 171.71) μm2 和单位平均面积 (2 9.81± 7.4 0 ) μm2 均较未表达组〔(3432 5 .6 5± 6 5 77.0 0 ) μm2 和 (37.84± 8.5 5 )〕μm2 显著降低 ,两组 IOD值无明显改变 ;Ki6 7和 Caspase3两组间无明显差异。结论 API2 - MAL T1表达与 API2蛋白的表达下调有关 ,API2的下调对 Caspase3影响不明显 。

Objective To investigate the expression of API2-MALT1 fusion gene mRNA and its effect on protein of API2, Caspase3 and Ki67 in gastrointestinal MALT lymphoma(Extranodal marginal zone B cell lymphoma of mucosa-associated lymphoid tissue). Methods The mRNA of API2-MALT1 fusion transcript was assayed in 33 archival cases of gastrointestinal MALT lymphoma by RT-PCR and nested PCR. According to the expression of API2-MALT1 mRNA or not, 33 cases of MALT lymphoma were divided into two groups. Ki67, API2 and Caspase3 were detected by immunohistochemical technique and analyzed by the microsoft Image-Pro plus. Results API2-MALT1 fusion gene mRNA was found in 16 of specimens with gastrointestinal MALT lymphoma. The expression rate was 48.4%. The average area (21177.30±6171.72) μm 2 and single cell average area (29.81±7.40)μm 2 of API2 were decreased in the group with the expression of API2-MALT1 mRNA, compared with those in the group without the expression of API2-MALT1 mRNA〔(34325.65±6577.00) μm 2,(37.84±8.55) μm 2〕. The integrated optical density(IOD) of API2 showed no significant difference between the two groups, neither did the expression of Ki67 and Caspase3. Conclusion The expression of API2-MALT1 is related to the down-regulated expression of protein of API2, its effect on apoptosis of MALT lymphoma remains to be detected further. The expression of API2-MALT1 mRNA is unrelated to the cellular proliferation.