横纹肌肉瘤脆性组氨酸三联体蛋白的表达

Fragile Histidine Triad Expression in Rhabdomyosarcoma

目的 探讨脆性组氨酸三联体 (FHIT)蛋白在横纹肌肉瘤 (RMS)中的表达及其在肿瘤发生发展中可能的作用机制。方法 应用免疫组织化学技术 (SP法 )检测 4 4例石蜡包埋人体横纹肌肉瘤细胞及 2 0例正常骨骼肌细胞中 FHIT蛋白的表达 ,并结合临床资料进行分析。结果 在 4 4例横纹肌肉瘤中 FHIT蛋白的低表达率为6 8.2 % (30 / 4 4 ) ,2 0例正常横纹肌的低表达率为 35 .0 % (7/ 2 0 ) ,两组间有显著性差异 (P<0 .0 5 )。患者不同性别、肉瘤组织学类型及分级间 FHIT蛋白的低表达率无显著性差异 (P>0 .0 5 ) ,但肿瘤的不同预后 (复发及转移 )间有显著性差异 (P<0 .0 5 )。结论 FHIT蛋白表达降低可能参与了横纹肌肉瘤的发生与发展 ,有望成为

Objective To study the expression of fragile histidine triad(FHIT) protein in rhabdomyosarcoma(RMS) and the possible mechanisms of its effect on tumor. Methods Immunohistochemical technique(SP) was used to detect the expression of FHIT in 44 cases of RMS and 20 cases of normal skeletal muscles. Results Reduced expression of FHIT was 68.2%(30/44) in the cases of RMS, and was 35%(7/20) in the cases of normal skeletal muscles; a significant difference was seen between the two groups(P<0.05). There was no significant relationship of the expression of FHIT with the sex of the patient and the histological type and grade of the tumor(P>0.05). However, a significant relationship was observed between the expression of FHIT and the prognosis of the tumor in respect to relapse and metastasis of tumor(P<0.05). Conclusion The reduced expression of FHIT may play an important role in the development and progression of rhabdomyosarcoma, and thus may become a new prognostic marker in RMS.