兔早期心肌缺血Connexin43的变化

Changes of Connexin 43 in Rabbit with Early Myocardial Ischemia

目的 探讨早期急性心肌缺血缝隙连接蛋白 4 3(Cx4 3)的变化。方法 结扎兔左冠状动脉造成急性心肌缺血模型 ,于结扎后 30 min取左心室前壁心肌 ,用免疫组织化学法和免疫电镜胶体金法观察、定位心肌缺血Cx4 3的分布 ,半定量统计分析。结果 急性心肌缺血 (30 m in) ,免疫组化图像分析系统分析结果显示 Cx4 3阳性面积减少约 4 0 % ;免疫电镜显示胶体金颗粒明显减少 ,分布未见改变。结论 兔急性心肌缺血早期 Cx4

Objective To reveal gap junction connexin 43 (Cx43) in rabbit with early myocardial ischemia. Methods The left coronary arteries were ligated to create early myocardial ischemia. Cx43 was visualized by use of SP immunohistochemical method(IHC) and Colloidal gold of the electron microscopy, and a comparison was made between the experiment group and the control group. The semi-quantitation assessment was performed. Results The positive areas of Cx43 gap junctions by IHC and Colloidal gold particles of the electron microscopy were found decreased by about 40% at 30 minutes of acute myocardial ischemia. The distributions were not obviously modified. Conclusion The decrease of Cx43 is characterized by the time during myocardial ischemia, and the early stage of acute myocardial ischemia in rat is of positive significance.