鼻咽低分化癌DNA含量及细胞周期分析

An Analysis of DNA Content and Cell Cycle in Nasopharyngeal Carcinoma

目的 探讨鼻咽低分化癌 DNA含量、细胞周期时相与临床分期的关系。方法 采用流式细胞术检测2 6例鼻咽低分化癌、6例鼻咽慢性炎症病变新鲜组织的 DNA含量及细胞周期各时相细胞成分。结果 6例鼻咽慢性炎症病变组织均为二倍体 ;2 6例鼻咽低分化癌中 19例 (73.0 8% )为异倍体 ;T2 、T3、T4 , 、 、 期鼻咽癌 ,其异倍体检出率分别为 5 5 .5 6 %、77.78%、87.5 0 % ;5 0 .0 0 %、75 .0 0 % ,77.78%。在鼻咽炎症及癌组织中 ,S期百分比(SPF)分别为 13.70 %、2 9.34% ;二倍体与异倍体鼻咽癌 ,其 SPF分别为 18.4 5 %、4 1.83% ;在二倍体 T2 、T3、T4 及 、 、 期鼻咽癌 ,SPF分别为 7.80 %、12 .70 %、32 .0 0 %及 13.85 %、31.4 3%、35 .30 % ;在异倍体 T2 、T3、T4 及 、 、 期鼻咽癌 ,SPF分别为 14 .0 4 %、17.5 9%、19.5 7%及 15 .10 %、2 3.33%、32 .2 8%。结论 鼻咽癌 DNA倍体类型以异倍体为主 ,增殖细胞群体以 S期细胞为主 。

Objective To evaluate the relationship between the DNA content, cell cycle and the clinical stages of nasopharyngeal carcinoma (NPC). Methods The DNA content and components of cell cycle in the fresh tissues of 26 cases of NPC and 6 cases of chronic pharyngitis were detected by flow cytometry. Results All the 6 cases of chronic pharyngitis were DNA diploid, 19(73.08%) cases of 26 NPC were aneuploid. The frequencies of aneuploid in NPC at T 2,T 3,T 4 and Ⅱ,Ⅲ,Ⅳ stages were 55.56%, 77.78%, 87.50% and 50.00%, 75.00%, 77.78%, respectively. S phase fractions (SPF) were 13.70% and 29.34% in pharyngitis and NPC respectively; 18.45%, 41.83% in diploid and aneuploid NPC respectively. In diploid NPC at T 2,T 3,T 4 and Ⅱ,Ⅲ,Ⅳ stages, SPF were 7.80%, 12.70%, 32.00% and 13.85%, 31.43%, 35.30%, respectively. In aneuploid NPC at T 2,T 3,T 4 and Ⅱ,Ⅲ,Ⅳ stages, SPF were 14.04%, 17.59%, 19.57% and 15.10%, 23.33%, 32.08%, respectively. There were significant differences in stastistics between the parameters. Conclusion Aneuploidy is the main characteristic change and S phase cells may constitute the main proliferating cell population in NPC, which increase with the advance of NPC.