产超广谱β内酰胺酶大肠杆菌的脉冲场凝胶电泳分型研究

Genotyping of EsBLs-producing Escherichia coli by Pulsed-field Gel Electrophoresis in Nosocomial Infection

目的 改进并运用基因分型方法 ,研究临床所分离到的产超广谱 β内酰胺酶 (Es BL s)大肠杆菌的分子流行病学。方法 应用 Es BL s表型确证试验检测产 Es BL s的大肠杆菌 ,分析病历资料以确定医院感染 ,采用脉冲场凝胶电泳 (PFGE)分析电泳图谱经数据转化后进行聚类分析 ,以确定菌株间的亲缘关系。结果 在重症监护病房分离到的 3株产 Es BL s的医院感染大肠杆菌 ,其 PFGE条带相似性达 95 %以上 ,提示为流行相关克隆。其他相似性系数在 5 0 %上的菌株由于不同条带均超过 6条 ,因而 ,被认为无流行相关性。结论 PFGE是一种用于产Es BL s的医院感染大肠杆菌分子流行病学研究的准确、可靠。

Objective Using a genotying method which is accurate and rapid to study the molecular epidemiology of EsBLs-producing Escherichia coli in nosocomial infection. Methods After an analysis of clinical information to ascertain nosocomial infection, EsBLs were detected by phenotype confirmation test and pulsed-field gel electrophoresis (PFGE). Results Three EsBLs-producing strains in ICU were verified as epidemiological-related because their dice coefficent index was higher than 95%. The others were verified as non-epidemiological-related since their different lanes were more than 6. Conclusion PFGE is a perfect molecular typing method to study epidemiolgy and analyze homology.