N-丁基壳聚糖氧氟沙星药物膜的制备及性能表征

Preparation and Characterization of the Membrane of N -Butylchitosan for Ofloxacin

15mL溴丁烷与4g壳聚糖在140mL异丙醇中60℃下搅拌反应5h制得N 丁基壳聚糖。以氧氟沙星为药物模型,制备了药物质量分数为0 5%,1 0%,2 0%和以N 丁基壳聚糖为涂层的药物膜,测定了膜的药物释放性能和力学性能。用红外光谱(FTIR)表征了膜中各组分的相互作用,扫描电子显微镜(SEM)表征了药物膜的形貌。结果表明,药物吸附在膜的表面,药物膜中氧氟沙星与N 丁基壳聚糖存在分子间的氢键,二者相互交联缠结形成半互穿网络。随着膜中药物含量的增加,药物膜的拉伸强度,断裂伸长率均下降,分别从28 4MPa降至20 2MPa,38 6%降至4 4%。与壳聚糖为载体的药物膜相比较,N 丁基壳聚糖为载体的药物膜有较高的断裂伸长率,为28.8%。药物膜中药物在pH=5 8的磷酸缓冲溶液中3h内释放率达99%,而涂层药物膜中药物在3h内释放率为88 0%,释放率达99%的时间为11h。

N -Butylchitosan was prepared by stirring 4 g chitosan and 15 mL butyl bromide in 140 mL isopropanol at 60 ℃ for 5 h.Using ofloxacin(FQ) as drug model,the membrane of N -butylchitosan containing FQ were prepared and coated with N -butylchitosan as the surface layer.The interaction of the components and the appearance and structure of membrane were investigated by FTIR and SEM.The results showed that there were hydrogen bond and semi-interpenetrating network between FQ and N -butylchitosan in the membrane and FQ was adsorbed on the surface of the membrane.With increase of the mass ratio of drug,tensile strength and break elongation of the membrane decreased from 28.4 MPa to 20.2 MPa and from 38.6% to 4.4% respectively.Compared with the chitosan-FQ membrane,the N -butylchitosan-FQ membrane had better mechanical properties and the break elongation was 28.8%.Release of FQ from the membrane reached 99% within 3 h in phosphate buffer solution of pH=5.8.However,the release of FQ from the coated membrane was 88% within 3 h and 99% within 11 h.