聚合酶链反应-单链构象多态性技术检测瘢痕疙瘩成纤维细胞p53基因突变

Study of p53 gene mutation in fibroblasts of keloids using PCR-SSCP

目的 :分析瘢痕疙瘩成纤维细胞p5 3基因突变高发区第 4～ 8外显子的突变及其意义。 方法 :取手术切除的瘢痕疙瘩和非增生瘢痕各 12例 ,分别采用相应正常皮肤对照 ,体外培养成纤维细胞 ,采用聚合酶链反应 单链构象多态性 (PCR SSCP)技术检测 p5 3基因的突变情况。 结果 :12例瘢痕疙瘩成纤维细胞标本中有 9例 p5 3基因外显子 4、5、6、7出现突变 ,非增生瘢痕标本和正常皮肤标本均未检出突变。 结论 :p5 3基因突变是瘢痕疙瘩形成和发展的重要因素之一。

Objective:To investigate the significance of exon 4-8 of p53 gene mutations in fibroblasts of keloids. Methods:Twelve keloid tissues, Twelve normal scar tissues and healthy skins were obtained from 24 patents with keloid or normal scar lesions. Using polymerase chain reaction-based single-stand conformational polymorphism (PCR-SSCP) to detect p53 gene mutations. Results:Mutations in the exon 4-7 of p53 gene were identified in 9 keloid fibroblast tissues. No p53 gene mutations were detected in normal scar and healthy skin samples of any of the patients. Conclusion:p53 gene mutations play an important role in the formation of keloids.