低强度氦氖激光照射对小鼠胸腺细胞体外增殖的影响

Influence of Low Energy He-Ne Laser Irradiation on Mouse Thymocytes Proliferation in vitro

目的 :研究低强度氦氖激光对体外培养的BALB/c小鼠胸腺细胞增殖的影响。方法 :采用MTT法检测不同功率密度、不同照射时间对不同起始培养密度小鼠胸腺细胞的增殖作用。结果 :低强度氦氖激光对小鼠胸腺细胞的增值有促进作用 ,当照射功率密度为 3.32 5mW /cm2 、照射时间 1～ 5分钟、起始细胞培养密度 2× 10 6个 /ml时效果最佳。结论 :低强度氦氖激光对小鼠胸腺细胞的增值有促进作用 ,促进小鼠胸腺细胞体外增殖的最佳条件为功率密度 3.32 5mW/cm2 、照射时间 1～ 5分钟、起始细胞培养密度 2× 10 6个 /ml,而照射时间超过 2

Objective: To investigate the influence of low energy He-Ne laser irradiation on mouse thymocytes proliferation in vitro. Methods: Using MTT assay to investigate the influences of different irradiation intensity, different irradiation time and different initial thymocytes density on the proliferation of thymocytes. Results: Low energy He-Ne laser irradiation has enhancing influences on mouse thymocytes proliferation in vitro. The most obvious effects can be observed when the irradiation power density is 3.325 mW/cm 2 , the irradiation time is 1～5 min and the initial thymocytes cultivation density is 2×106/ml. Conclusion: Low energy He-Ne laser irradiation has enhancing influences on mouse thymocytes proliferation in vitro. The optimal proliferation enhancement conditions are 3.325 mW/cm 2 irradiation power density, 1～5 min irradiation time and an initial thymocytes cultivation density of 2×106/ml, respectively. However, proliferation will be inhibited if the irradiation time exceeds 20 min.