免疫荧光染色检测不同组织细胞微管蛋白-β的表达

Tubulin-β Expression of Various Culture Cells Using Immunoinfluorescence

为了探讨体外培养的正常及肿瘤细胞微管蛋白 β的表达 ,将肺成纤维细胞 ( 3T3)、胃癌细胞 (BGC 82 3)、神经母细胞瘤株 (SH SY5Y)、大鼠胶质瘤细胞株 (C6)、多形性胶质母细胞瘤株 (BT32 5)体外培养 ,免疫荧光细胞染色检测微管蛋白 β的表达。发现 :所有细胞均为微管蛋白 β免疫反应阳性。结果提示 :微管蛋白

To explore tubulin-β expression of normal and tumor cells in vitro, 3T3, BGC- 8 23, SH-SY5Y, C 6, BT325 were cultured in vitro and immunofluorescent staining was used to investigate tubulin-β immunoreactivity. Results showed that the po sitive cells were intense red in body and processes which presented in all of ce lls. The findings prove that tubulin-β expression may occur in any multipl ication cell including normal cells and tumor cells.