外源性巯基物质抗氧化及对胰腺腺细胞保护作用的形态学观察

Antioxidant Effect of Exogenous Sulfhydyl Compound and Cytoprotective Effects on Pancreatic Acinar Cells in the Course of ANP

为观测急性坏死性胰腺炎 (ANP)时外源性巯基物质的抗氧化作用及其对胰腺腺细胞的保护作用 ,将雄性Wistar大鼠 (n =4 5)随机分成 3组 :A组 :ANP动物生理盐水治疗组 (n =18) ,B组 :ANP动物硫普罗宁治疗组 (n =18) ,C组 :假手术组 (n =9)。动物分别于手术后 4、6、12h时间点杀死 (A、B组各时间点各 6只 ,C组 3只 ) ,检测胰腺组织中丙二醛 (MDA)的质量摩尔浓度 ,并观察胰腺组织细胞的形态学变化。结果 :ANP时胰腺组织中MDA的质量摩尔浓度明显升高 ,外源性巯基物质能抑制腺组织中MDA的增加 ;ANP时胰腺组织细胞损伤严重 ,外源性巯基物质能减轻胰腺组织细胞的损伤 ,尤其能减轻腺细胞粗面内质网的损伤。提示 :ANP时外源性巯基物质具有显著的抗氧化作用 ,并能保护胰腺组织细胞 。

Objective: To observe the antioxidant effect and the cytoprotective effect from morphological point of view of exgenous sulfhydryl compound on pancreatic acina r cells in the cou rse of acute necrotic pancreatitis. Methods: Male Wistar mice were divided rand omly into three groups: Group A and B served as animal models, A ( n =18) with normal saline therapy, whi le B ( n =18) with Tiopronin therapy; and group C ( n =9) a normal control. Animals were killed 4, 6 and 12 h after operation respectively. Malondiadehyde(M DA) was analyze d and the pancreatic acinar cells were observed histologically under light mic roscope and electromicroscope. R esults: The level of MDA increased highly during the course of ANP, and it was p revented obviously when Tiopronin-an exgenous sulfhydryl compound was administr ated prophylactically. The pancreas from rats of group A suffered from acute necroti c pancreatitis (ANP). Morphological changes were profound in the pancreatic tiss ue and the acinar cells, and the damage to the rough endoplasmic reticulum (RER) and the mitochondria was most serious. The damage was attenuated to a certain e xtent especially when the RER of acinar cell with Tiopronin was used. Conclusion : A disti nctly antioxidant effect of exgenous sulfhydryl compound is found in the course of ANP, a nd it could protect the acinar cells especially the ultrastructure of the RER.