大肠癌组织端粒酶活性检测

Detection of Telomerase Activity in Tissues of Colorectal Carcinomas

为研究大肠癌组织中端粒酶活性的表达情况 ,探讨其在大肠癌预防、诊断、治疗方面的临床价值。采用端粒重复扩增法 (TRAP)检测 4 0例大肠癌组织及其中 10例癌旁正常黏膜组织的端粒酶活性。发现 :4 0例大肠癌组织中 ,34例端粒酶活性阳性表达 ,阳性率为 85% ,10例癌旁正常黏膜组织端粒酶活性表达均为阴性。 2组阳性率比较差异有显著性 (P <0 .0 5)。端粒酶活性与大肠癌部位、细胞分化程度、Dukes分期及是否伴有淋巴结转移无关 (P >0 .0 5)。提示 :端粒酶普遍存在于大肠癌组织中 ,可作为大肠癌的基因标志物。检测大肠病变组织端粒酶活性有可能对大肠癌的早期发现。

The aim was to study the expression of telomerase activity in tissues of colorec tal carcinom a and to investigate its clinical value in prevention, diagnosis, treatment. A t otal of 50 human colorectal tissue specimens, comprising 40 carcinomas and 10 tu mor-adjacent normal tissue specimens were examined for telomerase activity by t elomeric repeated amplification protocol (TRAP) assay. In the 40 colorectal carc in oma specimens, 34 cases were detected positive expression of telomerase activity (85%), 10 specimens of tumor-adjacent normal tissue were negative. There was si gnificantly difference between the two groups ( P < 0.05). There was no rel ationshi p between telomerase activity and the site of colorectal carcinoma, the level of cell differentiation, Dukes stage, metastasis of lymph nodes ( P > 0.05 ). Th ere is common telomerase activity of colorectal carcinoma tissues. Telomerase ac tivity can become a gene marker of colorectal carcinoma tissues. Detecting the t elomerase activity of colorectal carcinoma tissues may help early discovery, ear ly diagnosis, early treatment. Inhibiting the telomerase activity may cure color ectal carcinoma. Inhibiting or destorying the process of telomerase activity may prevent the cause of colorectal carcinoma.