链球菌组蛋白样蛋白对小鼠血清IL-6质量浓度的影响

Effect of Streptococcal Histone-Like Protein on IL-6 Level in Murine Serum

为观察链球菌组蛋白样蛋白 (HlpA)对小鼠血清白细胞介素 6 (IL 6 )质量浓度的影响 ,经小鼠尾静脉注射HlpA ,用 7TD1IL 6依赖株的细胞存活率测定小鼠血清IL 6质量浓度。发现 :链球菌HlpA( 0～ 30 0 μg/只 )以剂量和时间依赖方式促进小鼠血清IL 6升高 (P <0 .0 5～P <0 .0 1)。脂磷壁酸 (LTA)和HlpA有协同增强作用 ;抗HlpA和肝素则对HlpA起抑制作用。提示 :纯化的链球菌HlpA刺激小鼠血细胞过量产生IL 6 ,LTA对此有增强作用 。

The goal was to evaluate the effect of Streptococcal HlpA on IL-6 level in muri ne serum. Serum levels of IL-6 production were measured by means of 7TD1 IL-6 dependent cell line. The IL-6 levels in murine serum induced with Streptococcal HlpA were time and concentration dependent in vivo. There was a synergistic res ponse on the IL-6 level in murine serum induced by the mixture of HlpA and LTA, compared to HlpA alone. On the contrary, anti-HlpA and heparin could restrain serum levels of IL-6 production induced by intravenous injection o f HlpA. It means that Streptococcal HlpA can increase serum levels of IL-6 pr oduction induced by intravenous injection of HlpA. There was significant synergi sm on it in mice vivo after HlpA and LTA were injected together.