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培养原始生殖细胞的新方法——Sertoli细胞的应用 被引量:5

New Culture Method for Primordial Germ Cell——Application of Sert oli Cells
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摘要 为探讨睾丸支持细胞 (sertolicell,SCs)对原始生殖细胞 ( primordialgermcell,PGCs)的增生与生长是否有明显的促进作用。用SCs和同源生殖嵴成纤维细胞分别与PGCs共培养。结果发现 :与SCs共培养的PGCs集落多于与同源生殖嵴成纤维细胞共培养的PGCs集落 ,传代次数也多于同源生殖嵴成纤维细胞。提示 The aim was to investigate the effects of sertoli cells (SCs) on the growth an d proliferatio n of primordial germ cells (PGCs). Primordial germ cells were co-cultured with homologue fibroblasts from genital ridge or with sertoli cells. Result: When co -cultured with SCs, PGCs colonies and times of passage were greater than those co-cultured with homologue fibroblasts. Conclusion: Sertoli cells can enhance p rimordial germ cells' proliferation in cell culture.
出处 《首都医科大学学报》 CAS 2004年第1期15-18,共4页 Journal of Capital Medical University
基金 北京市教委科技发展计划 (KM 2 0 0 3 10 0 2 50 89)资助项目
关键词 原始生殖细胞 SERTOLI细胞 细胞培养 增生能力 sertoli cells cell culture primor dial germ cells
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参考文献12

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