胆管癌及癌旁组织中端粒酶逆转录酶蛋白和基因的表达及意义

The expression and significance of human telomerase reverse transcriptase protein and gene in bile duct carcinomas and their adjacent tissues

目的 检测胆管癌和癌旁胆管组织中端粒酶逆转录酶 (hTERT)蛋白和基因的表达 ,探讨其在胆管癌发生中的作用。方法 应用免疫组化S P法检测 71例胆管癌和 39例癌旁胆管组织中hTERT蛋白的表达 ,同时应用组织原位杂交技术检测这些组织标本中hTERTmRNA的表达 ,并与临床病理资料进行相关性分析。结果 71例胆管癌组织中 ,hTERT蛋白阳性表达率为 78.9% (5 6 /71) ,hTERTmRNA阳性表达率为 6 7.6 % (4 8/71) ;而 39例癌旁胆管组织中的hTERT蛋白阳性表达率仅为35 .9% (14 /39) ,hTERTmRNA阳性表达率仅为 2 3.1% (9/39) ,且阳性信号全部见于伴有不典型增生的胆管上皮细胞内。病理资料的相关性分析显示 ,胆管癌组织中 ,hTERT蛋白和基因的表达分布与临床病理特征无相关性。结论 hTERT基因转录和蛋白表达可能参与胆管癌的发生发展过程 ,检测hTERT表达可能有助于阐明胆管癌的发生机制。

Objective To detect the expression of human telomerase reverse transcriptase(hTERT) protein and mRNA in bile duct carcinomas and the adjacent tissues and to elucidate its role in bile duct carcinogenesis. Methods The expression of hTERT protein and hTERT mRNA in the formalin-fixed paraffin-embedded specimens of 71 cases of bile duct cancers and 39 cases of adjacent tissues was detected by streptavidin-peroxidase immunostaining and in situ hybridization. The correlation was analysed statistically between the expression of hTERT protein and mRNA and clinicopathological parameters bile duct carcinomas. Results The positive rate of hTERT protein expression and mRNA expression in malignant specimens was 78.9%(56/71) and 67.6%(48/71),while that in the adjacent tissues was 35.9%(14/39) and 23.1%(9/39), respectively. All the positive signals were found in the hyperplastic biliary epithelia. No significant correlation was established between hTERT expression and clinicopathological parameters. Conclusion hTERT gene transcription and protein expression is most likely involved in the proliferation and malignant transformation of bile epithelia and the malignant progression of bile duct carcinomas. The detection of hTERT expression may serve elucidating the carcinogenesis of bile duct.