胃异型增生上皮、胃癌活检组织p16和细胞周期蛋白D_1的检测

The detection p16 protein and cyclinD_1 in gastric epithelial dysplasia and adenocarcinoma

目的 检测胃异型增生上皮及胃腺癌组织中p16蛋白和细胞周期蛋白D1(cyclinD1)的表达。方法 应用免疫组织化学S -P法检测 49例低度异型增生、16例高度异型增生及 3 0例胃腺癌活检组织中p16蛋白和细胞周期蛋白D1的表达。结果 p16蛋白和细胞周期蛋白D1在低度异型增生、高度异型增生及胃腺癌活检组织中阳性表达率有显著性差异 (P <0 .0 1)。低度异型增生和高度异型增生p16蛋白和细胞周期蛋白D1的表达率与是否伴有肠化无关。结论 p16蛋白和细胞周期蛋白D1可作为判断胃异型增生上皮生物学行为有价值的指标。

ObjectiveTo detect the p16 protein and cyc linD 1 in gastric epithelial dysplasia and adenocarcinoma.Methods The expressions of p16 protein and cyclinD 1 in 49 low grade epithel ial dysplasia , 16 high grade epithelial dysplasia, and 30 gastric adenocarcinom as obtained by endoscope were detected by Immunohistochemical S-P method.Resu ltsThe positive expression rates of p16 protein and cyclinD 1 had significant difference(P<0.01) in three groups. The positive expre ss ions of p16 protein and cyclinD 1 were not closely related to intestinal metapl asia in low grade epithelial dysplasia and high grade epithelial dysplasia.Con clusionp16 protein and cyclinD 1 may be valuable marker s in assessing biological behavior of gastric low grade epithelial dysplasia an d high grade epithelial dysplasia.