离子注入与γ射线对水稻诱变效应的初步研究

Mutagenic Effects of Ion Implantation and Gamma-rays in Rice

用水稻干种子作为诱变材料,以M_1代成苗率、苗高、株高和结实率为生理效应指标,以M_2代叶绿素突变频率为诱变效应指标,研究了离子注入和γ射线的生理效应和诱变效应。结果表明,离子注入对水稻的损伤较γ射线处理轻。在适宜剂量下,离子注入的M_2代叶绿素突变率高于γ射线处理。用于水稻干种子诱变时离子束的适宜注入剂量为6-8×10^(16)N^+/cm^2。

Physiological and mutagenic effects of ion implantation and gamma-rays on rice dormant seeds were studied, using seedling survival, seedling height, plant height and fertility of M, plants and the frequency of chlorophyll mutation in M_2 generation as criteria. The results indicated that ion implantation showed less physiological damage than gamma-rays. The frequency of chlorophyll mutation in M_2 generation treated with ion implantation was found to be higher than that treated with gamma—rays when the optimal dose of ion implantation was applied. The appropriate dose of ion implantation was 6-8 x 10^(16) N^(+)/cm^2 for rice dormant seeds.