DNA微阵列图象信息的自动提取

Automatic-Extracting Information from DNA Microarray Images

微阵列图象分析是分子生物学中DNA微阵列杂交实验数据测定过程的一个重要部分。其目的是将微阵列图象中大量象素灰度信息简化为微阵列靶点的信号值。该文介绍了一种对DNA微阵列图象信息进行自动提取的方法,使用此方法完全不需要人为操作,可避免操作者主观性对实验的影响,提高数据提取的效率和可重复性。其关键步骤包括:图象滤波,图象灰度信息提取,微阵列间距确定,靶点定位和靶点信号值计算。实验结果表明使用此算法提取DNA微阵列图象信息具有重复性好、效率高和分析准确的特点。

The goal of DNA microarray image analysis is to reduce the large number of pixel values in the image file to a small set of summary values representing each printed spot on the array.In this paper,an effective algorithm for automatic-extracting information from DNA microarray images is introduced,which can extract information without user intervention.This algorithm includes image filtering,extracting gray information,determining microarray spacing,addressing of spots and calculating brightness property from spots.The result of experiment shows that the algorithm is efficacious.