两种去细胞处理猪皮与微粒皮复合移植对创面愈合的影响

The Influence of Composite Transplantation of Two Kinds of Acellular Xeno-dermis and Micro-autograft on Wound Healing

目的 研究自体微粒皮与异种 (猪 )去细胞真皮基质及选择性去细胞异种 (猪 )皮复合移植对创面愈合的影响。方法 以 72只SD大鼠为动物模型分为复合皮组I、复合皮组II和微粒皮组 ,复合皮组I于背部全层皮肤缺损创面移植异种 (猪 )无细胞真皮 +自体微粒皮 +选择性去细胞异种 (猪 )皮 ,复合皮组II移植异种 (猪 )无细胞真皮 +自体微粒皮 +戊二醛异种 (猪 )皮 ,微粒皮组移植自体微粒皮。术后定期观察创面愈合情况 ,计算创面收缩率和创面愈合率 ,取活检标本行组织学、免疫组化及电镜观察。结果 经组织学 ,免疫组化染色及电镜观察 ,选择性去细胞异种 (猪 )皮表皮层连续、完整 ,真皮中三大纤维排列有序且无细胞成分 ,基底膜结构完整 ;复合皮组I上皮化良好 ,胶原纤维排列有序 ,免疫组化染色证实有完整的基底膜生成。复合皮组I的创面愈合效果优于复合皮组II及微粒皮组。结论 选择性去细胞异种 (猪 )复合皮 (异种无细胞真皮 +自体微粒皮 +选择性去细胞异种皮 )

Objective To investigate the influence of composite transplantation of two kinds of acellular xeno-dermis (pigskin) and micro-autograft on wound healing. Methods Seventy-two Sprague-Dawley rats with full-thickness skin defect on the back were randomly divided into three groups, and then compositt skin I(acellular dermal xeno-matrix+autologous particulate skin +selective acellular xeno-dermis) was adopted to cover the skin defect in the experimental group I, composite skin II(acellular dermal xeno-matrix+autologous particulate skin + glutaraldehyde xeno-skin) was adopted to cover the skin defect in the experimental group II, and auto-particulate skin was adopted in the control group. The area of wound healing and the rates of wound contraction were calculated.Tissue samples were harvested and examined by means of histology, immunohistochemistry and electron microscopy. Results There were continuous epidermis and basement membrane, and the dermal matrix was a kind of matrix with normal structure and organization of collagen and without any cellular components in selective acellular xeno-dermis. There was full differentiation of epithelium, orderly collagen arrange and basal membrane of the skin could be identified by immunohistochemical stain in compound skin grafting group I. The healing effect of compound skin grafting group I was better than compound skin grafting group II and auto-particulate skin grafting group. Conclusion The composite grafts constructed by selective acellular xeno-dermis (pigskin) could be a potential new type of composite skin substitute for the repair of full-thickness skin defect.