摘要
目的 研究中药MBC提取物在体外对K5 62 /ADM逆转作用及机制。方法 应用MTT法测定应用MBC前后ADM对K5 62 /ADM耐药细胞株的IC50 ,计算其逆转倍数。应用流式细胞仪技术测定应用MBC前后细胞内Rh12 3浓度。结果 8× 10 -1 μg/mlMBC能增加ADM对K5 62 /ADM的细胞毒作用 ,逆转倍数 6 2 8倍 ,应用MBC后K5 62 /ADM细胞内Rh12 3浓度增加。结论 MBC可提高ADM对耐药细胞K5 62 /ADM的杀伤作用 ,部分逆转了K5 62
Objective To investigate the reverse effect and mechanism of the extracted solution of chinese medicine MBC on the drug-resistance of K562/ADM in vitro. Methods MTT assay was used to determine the IC50 of ADM on K562/ADM cell line and the reverse multiples of the drug-resistance before and after MBC treatment. Flow cytometry(FCM) was applied to assess the intracellular concentration of Rh123. Results 8×10 -1 μg/ml MBC increased cytotoxic effect of ADM on 562/ADM cells, the reverse multiple was 6 28, and MBC could increase the intracellular concentration of Rh123. Conclusion MBC could increase killing effect of ADM on the drug-resistant K562/ADM cell and partly reverse the drug-resistance of K562/ADM cell.
出处
《中国医师杂志》
CAS
2004年第3期307-309,共3页
Journal of Chinese Physician
基金
吉林省科技厅资助重点课题项目 (2 0 0 0 0 30 3)
