转化生长因子β与硬皮病临床分类的关系

The Relationship Between the Expression of TGF-β and the Types of Scleroderma

目的 通过对SD患者皮损中TGF -β1 、β2 mRNA和蛋白表达水平的检测 ,探讨TGF -β1 、β2 mRNA和蛋白在SD的发病机制中的作用 ,探讨TGF -β1 、β2 mRNA和蛋白与SD临床分类间的关系。方法 采用原位逆转录聚合酶链反应 (原位RT -PCR)技术和免疫组化SP方法 ,检测 17例SD患者皮损中TGF -β1 、β2 mRNA和蛋白的表达情况 ,并以 10例正常皮肤组织作为对照。结果 ①TGF -β1 mRNA在SD组表达阳性率、表达程度和积分值高于对照组 ;但TGF -β1 mRNA在局限性SD组和SSc组表达阳性率相近。②TGF -β1 、β2 蛋白在SD组表达阳性率、表达程度和积分值均明显高于对照组 ;而TGF -β1 、β2 蛋白在局限性SD组和SSc组表达阳性率均为 10 0 %。结论 ①SD组TGF -β1 mRNA表达阳性率和表达程度均增高 ,表明TGF -β1 mRNA在SD纤维化过程中起重要作用。②SD组TGF -β1 、β2 蛋白表达阳性率和表达程度均明显增高 ,显示TGF -β1 、β2 蛋白与SD皮肤纤维化有密切关系。③TGF -β1 、β2mRNA和蛋白的表达与SD的临床分类无相关性 。

Objective To investigate the role of TGF-β 1 and -β 2 in the pathogenesis of scleroderma(SD) and the relationship between this kind of cytokines and the clinical types of SD. Methods The TGF-β 1 and -β 2 mRNA and protein expressions in lesions of 17 patients with SD and 10 normal controls were detected using in situ RT-PCR technique and immunohistochemistry SP assay respectively. Results ①The positive rate and the expression strength of TGF-β 1 mRNA expression in the SD group were much higher than those in control group. There was no difference in TGF-β 1 mRNA expression between the localized SD and SSc patients. ②The positive rate and expression strength of TGF-β 1 and -β 2 proteins in SD group were much higher than those in control group. There was no difference in TGF-β 1 and -β 2 protein expressions between localized SD and SSc cases. Conclusion ①The positive rate and expression strength of TGF-β 1 mRNA in SD patients increased, which implied that TGF-β 1 mRNA may play an important role in fibrosis of SD. ②The positive rate and expression strength of TGF-β 1 and -β 2 proteins were more elevated in SD,which suggested that TGF-β 1 and -β 2 proteins were associated with skin fibrosis of SD. ③There was no relationship between the expression of TGF-β 1 and TGF-β 2 mRNA or proteins and the clinical types of SD, which indicated that there may be a similar pathogenesis for localized SD and SSc.