通肾汤对阿霉素肾病综合征大鼠肾小球基底膜的影响

Effect of Tongshen Decoction on Glomerular Basement Membrane in Rats with Adriamycin-Induced Nephrotic Syndrome

【目的】探讨通肾汤治疗肾病综合征的作用机理。【方法】采用阿霉素肾病综合征大鼠模型及多聚亚胺染色方法,检测肾小球基底膜(GBM)上的阴离子位点数。【结果】正常大鼠GBM上阴离子位点分布以外疏松层为主;阿霉素肾病综合征模型大鼠GBM上阴离子位点较正常组减少(P<0.01),外疏松层减少更为明显;通肾汤能够使GBM阴离子位点恢复正常,与阿霉素肾病综合征大鼠模型组比较具有显著性差异(P<0.05),与强的松比较无显著性差异(P>0.05);两药合用作用更明显。【结论】通肾汤能保护肾小球滤过膜的电荷屏障,其治疗肾病综合征的作用可能与此有关。

[Objective] To explore the therapeutic mechanism of Tongshen Decoction (TD) on glomerular basement membrane (GBM) in rats with adriamycin-induced nephrotic syndrome. [Methods] Forty SD rats were randomized into normal group (A), model group (B), prednisone group (C), TD group (D) and prednisone combined with TD group (E). Rat model of adriamycin-induced nephrotic syndrome was applied and glomerular anionic sites were observed by polyimine staining method. [Results] Glomerular anionic sites mainly distributed in the exterior loose connective tissues in group A. The number of glomerular anionic sites in GBM of group B was decreased as compared with that in group A (P<0.01), especially in the exterior loose connective tissues. TD could raise the number of glomerular anionic sites to normal level, the difference being significant as compared with group B while being insignificant as compared with group C. The effect of TD combined with prednisone was more stronger than TD or prednisone only. [Conclusion] The possible mechanism of TD in treating adriamyein-induced nephrotic syndrome may be related to the protection of charge barrier in GBM.