实验性脑积水轴索细胞骨架的变化

Changes of axonal cytoskeleton of experimental hydrocephalus

目的 探讨脑积水中轴索损伤的发展变化规律。方法 以枕大池穿刺注射白陶土的方法建立犬脑积水模型 ,分为 3d组、2周组、8周组及对照组 ,每组 5只。通过免疫组化方法观察半球白质内轴索的形态变化 ,并使用电脑图像分析系统定量测定轴索细胞骨架的结构亚单位微管相关蛋白 2 (MAP 2 )和神经微丝 (NF)蛋白亚单位含量。结果 ①轴索随脑积水发展相继出现扭曲、肿胀、断裂以及轴索球形成等形态学改变 ;② 3d组MAP 2含量与对照组相比无显著差异 (P >0 .0 5 ) ,NF蛋白有下降 (P <0 .0 5 ) ,在 2周组和 8周组 ,两者则进行性减少 (P <0 .0 5 )。结论 脑积水中的轴索损伤是以细胞骨架破坏为起始的一个渐进过程 ,它在早期阶段是可逆的 ,通过综合治疗有望阻断这一过程。

Objective To explore the regularity of axonal injury in experimental hydrocephalus.Methods 20 male adult dogs were induced hydrocephalus by injection kaolin into cisterna magna, then divided into 4 groups according to the time being killed(5 in each): 3 days group, 2 weeks group, 8 weeks group and control group. Imunohistochemistry was performed to show the morphological changes of axon in white matter. Furthermore, the content of protein subunit of axonal cytoskeleton, including microtubule associated protein-2(MAP-2) and neurofilament (NF) protein, was determined quantitatively with a computer image analysis system.Results ①With the development of hydrocephalus, the morphological changes of axon included distortion, swelling, disconntection and formation of axonal bulbs etc. ②Compared with control group, the content of MAP-2 had not statistical difference in 3 days group(P>0.05), while NF protein decreased(P<0.05). In 2 weeks and 8 weeks group, both of them came down progressively(P<0.05).Conclusion Axonal injury in hydrocephalus is a gradual progress beginning at destruction of cytoskeleton, which is reversible at early stage. It is hopeful that this course can be interdicted by comprehensive therapeutics.