摘要
研究了表面活性剂和超声波对大肠杆菌AS1 .881中天冬氨酸酶活力的影响。结果显示 ,2 -YT培养基提取的天冬氨酸胞内酶 ,用表面活性剂 (曲那通、十二烷基硫酸钠等 )破壁 ,游离细胞的最高酶活力由报道的 1 6万单位每克湿细胞提高到31 .3万单位每克湿细胞。表面活性剂最佳质量浓度范围为 0 .1 6 %～ 0 .5 8%。在 7KHz超声波强度下破壁 5min ,游离细胞的最高酶活力也能提高到 30万单位每克湿细胞 ,证明表面活性剂和超声波处理胞内酶 ,破壁效果好 。
The effect of surfactant and ultrasonic wave on aspartase in E.coli was investigated. The results indicated that the activity of aspartase was up to 313000 u/g wet cell by using the surfactant, exceeding 160000 u/g wet cell reported in the literatures. The optimal range of surfactant concentration was 0.16% to 0.58%. The enzyme activity of aspartase was up to 300000u/g wet cell by using the ultrasonic wave. The conditions were 7 KHz in intensity and 5min in time respectively.
出处
《氨基酸和生物资源》
CAS
2004年第1期29-31,37,共4页
Amino Acids & Biotic Resources
基金
八峰药化股份有限公司资助课题
