摘要
目的:探讨大黄蒽醌类抑制大肠杆菌内毒素(escherichia coli lipopolysaccharide E-LPS)对人牙周膜细胞(periodontal ligament cells,PDLC)的作用。方法:采用原代培养人PDLC技术和四唑盐(MTT)显色分光光度法。结果:20,50 μg/ml大黄素明显抑制E-LPS对人PDLC的影响,与对照组相比有显著性差异。结论:大黄素能阻断内毒素对人PDLC的毒性。提示应用大黄素治疗牙周炎在临床上可行。
Objective: To investigate the effects of rhapontic arthraquinone ramification (RAR) to inhibit the poison of escherichia coli lipopolysaccharide (E - LPS) to human periodontal ligament cells( PDLC). Method: Using in vitro human periodontal ligament cells culture technique and thiazolyl blue tetrazolium bromide (MTT) staining and spectrophotornetric assay. Result: 20,50 μg/ml emodin obstruct the inhibiting of E- LPS to human PDLC . Conclusion:Emodin can be used to treat periodontal diseases and have clinical value.
出处
《临床口腔医学杂志》
2004年第3期148-150,共3页
Journal of Clinical Stomatology
基金
南京市科技发展基金(ZKX9817)
关键词
大黄
内毒素
牙周膜细胞
细胞培养
rhubarb
lipopolysaccharide
periodontal ligament cells
cell culture