P53基因对K562细胞增殖的影响

Effects of p53 gene on K562 cell line proliferation

目的 观察恢复K5 6 2细胞的p5 3蛋白功能后该细胞的生物学行为的改变 ,探索用野生型p5 3基因治疗白血病的可能性。方法 以K5 6 2细胞为研究对象 ,电穿孔法转导野生型p5 3基因 ,RT PCR和免疫细胞化学方法检测p5 3基因的表达 ,3H TdR掺入法检测细胞增殖 ,用流式细胞仪分别以TUNEL、annexin V、细胞周期检测细胞的凋亡情况和细胞周期分布情况。结果 转染p5 3基因的K5 6 2细胞 ,出现明显细胞周期G1 期停滞 ,增殖抑制率为 2 4 .17% ,但用TUNEL、annexin V和亚二倍体峰检测未发现与未转染p5 3基因组有凋亡细胞比例差异。结论 P5 3基因对K5 6 2细胞有一定的治疗效应 。

Objective To investigate effects of p53 gene on proliferation and apoptosis in K562 cell line. Methods P53 gene was transferred to monoclonal K562 cells with electroporation. P53 gene transcription and expression were detected with RT- PCR and immunocytochemical staining. Proliferation test was conducted by using 3 H-thymidine ( 3 H-TdR) incorporation assay, and apoptosis was analyzed by TUNEL, annexin V and hypodiploid apex. Results Compared with wild type K562 and neo-K562, proliferation of p53-K562 decreased by 24.17% and exhibited G 1 arrest. Significant apoptosis did not occur. Conclusion P53 gene could induce less proliferation and G1 arrest but not apoptosis.