摘要
针对鸡毒霉形体 (MG) 16S和 2 3SrRNA基因间的高变区序列设计合成了 1对引物 ,对MG菌株进行了PCR扩增 ,获得了 1条 899bp的DNA片段 ,而其他禽病病原DNA无扩增。扩增产物经用限制性内切酶AfaⅠ和DraⅠ进行酶切片段长度多态性分析 ,表明各MG菌株酶切图谱间均存在差异。
According to Mycoplasma gallisepticum (MG) hypervariable region between 16 S rRNA and 23 S rRNA, a pair of primers was designed. The MG DNA fragment 899 bp in length was specifically amplified by means of using the primers. The PCR product was analysed by the restriction fragment length (polymorphism) (RFLP) method. The results indicated that the RFLP patterns of the amplified fragment were different. The current study may play important role in MG epidemiological investigation in Guangxi Region.
出处
《中国兽医科技》
CSCD
北大核心
2004年第3期41-44,共4页
Chinese Journal of Veterinary Science and Technology
基金
广西留学基金 (桂科回 0 0 0 90 0 1)
关键词
鸡毒霉形体
PCR
限制性片段长度多态性
广西
PCR
restriction fragment length polymorphism (RFLP)
Mycoplasma gallisepitcum
