非致病性镰刀菌Fusarium oxysporum菌株47(FO47)对番茄枯萎病的防治效果

Biocontrol Effect of Non-pathogenic Fusarium Oxysporum Strain 47 (FO47) Against Fusarium Wilt of Tomato

本实验对非致病镰刀菌Fusariumoxysporum菌株47号(简称FO47)对番茄枯萎病的生物防治效果进行了初步研究。通过对峙培养和多孔细胞培养板测试以及室内盆栽试验表明,FO47在对峙培养平板上对番茄枯萎病菌无拮抗作用;施用FO47的最佳时机是番茄播种至子叶平展期,最佳施用浓度为105～108分生孢子/毫升(简写为cfu·ml-1),防效为63.6%;结果还表明FO47液体培养的过滤液能有效抑制番茄枯萎病菌孢子的萌发,抑制萌发率为81.7%。

Non pathogenic Fusarium oxysporum strain 47 (FO47) was studied for its biocontrol effect against Fusarium wilt of tomato. Through the co-culture on PDA plates, multi-well cell culture plates test and the experiment at seedling stage, the results showed that the FO47 had no direct antagonistic effect against the pathogenic fungus; The results of experiment at seedling stage showed that best time for controlling the disease was from the sowing stage to cotyledon stage and that best concentration was from 105 to 108 cfu·ml-1 and the best control effect was 63.6 %. The filtrate of FO47 liquid culture could inhibit spore germination and could affect the mycelium growth of the pathogen.