三种木本植物基因组DNA的提取及纯度检测

The Extraction and Purity Characterization of Genome DNA From Leaf-tissue of Three Ligneous Plants

木本植物体内含有大量的酚类、多糖等次生代谢物质,严重影响提取其基因组DNA的得率和纯度.本文分别用改进的高盐低pH法、2×CTAB法和SDS法从脱皮榆、酸枣、翅果油树的叶组织中提取基因组DNA,通过琼脂糖凝胶电泳和紫外分光光度法检测所提取基因组DNA的纯度,分别筛选出简便、经济、适合脱皮榆、酸枣、翅果油树基因组DNA提取的方法,为其分子生物学研究及下游操作奠定了基础.结果也表明抗坏血酸、PVP、β-SH等抗氧剂为木本植物基因组DNA提取所必须的.

There are a mass of phenolic compound, polysaccharides and other secondary metabolites in ligneous plant, which influence the yield and quality of genome DNA while being extracted. A set of method of extracted genome DNA was studied for U.Lamellosa, E. mollis, Z. Jujuba respectively from the leaf tissue according as low PH with high salts extraction medium, 2×CTAB and SDS.The results of extracted genome DNA purity was characterised through Agrose Gel Electrophoresis and UV-vis Recording Spectrophotometer. The results showed 1) that improved 2×CTAB adapt to U.Lamellosa, Z. Jujuba and improved SDS to E. mollis which is respectively rapidly, economical method, 2) that ascorbin acid, poly vinylpyrrilodone andβ-SH are pre-requisite for the ligneous plants. The foundation for further study of molecular biology of each species is importance.