摘要
对麦红吸浆虫Sitodiplosismosellana(Gehin)11个地理种群线粒体DNA(mtDNA)细胞色素C氧化酶亚基II(COII)的部分片段进行了测序,分析了核苷酸的组成,构建了NJ分子系统树。结果表明,在用于分析的447bp序列中,A+T平均含量为78.6%,其中7个位点存在变异。NJ树显示,麦红吸浆虫各种群间的亲缘关系与地理分布关系密切:11个地理种群可明显地聚为两大族群,所有来自春麦区的种群(NH、QX、GT、GW、GG)和冬麦区(陕西长安SC)种群聚为族群Ⅰ,其它冬麦区种群(HT、HL、HX、AS、AF)聚为族群Ⅱ;陕西长安种群(SC)虽处于冬麦区,但与春麦区种群具有很高的遗传相似性。该结果支持将我国麦红吸浆虫的发生区域分为三个,即春麦发生区、冬春麦发生区、冬麦发生区。各地理种群间的遗传距离为0～0.016,表现为种下的遗传变异,这与RAPD分析得出的结果相一致。
Mitochondrial DNA COII sequences (447bp) of geographical populations of Sitodiplosis mosellana were sequenced from 11 different areas of China .Their nucleotide compositions were analyzed and a molecular phylogenetic tree was constructed with the Neighbor-Joining method. Results showed that in the sequences obtained, mean A+T content was about 78.6% and 7 nucleotide sites were substituted. The NJ tree suggested that the phylogeny among populations of Sitodiplosis mosellana is related closely with geographical distribution, 11 populations are clustered into 2 groups. Group I includes all the spring wheat region populations (NH, GW, GG, QX, GT) and SC population; group II includes all the winter wheat region populations (HL, HX, HT, AS, AF), But SC population has high similarity with spring wheat region populations. This result supports the view that the distribution of Sitodiplosis mosellana could be divided into three groups: spring wheat region , winter wheat region ,and mixture region of winter and spring wheat. Genetic distance among different populations are 0～0.016, which is belong to infraspecies genetic variation, this result is consistent with RAPD analysis.
基金
国家自然科学基金资助项目(39770499)
