磺胺二甲嘧啶对大鼠早期身体及智力发育的影响

The Effects of Exposure to Sulphamethazine on the Early Development of Body and Intelligence of Rats

目的研究受孕中晚期及哺乳期母体接触磺胺二甲嘧啶对子代大鼠早期身体及智力发育的影响。方法受孕母鼠在受孕第7天起至哺乳期结束分别给予磺胺二甲嘧啶0、50、100、200mg/(kg·d),观察F1代大鼠体重及开眼时间,测定各组出生后不同天龄大鼠的血清游离T4及TSH水平,并用水迷宫实验检测各组20～30天龄大鼠的学习和记忆功能。结果实验组F1代大鼠血清游离T4水平低于对照组,同时发现实验组F1代大鼠体重降低,开眼延后,以及学习记忆功能障碍,均表现出剂量-反应关系,尤其是100、200mg/kg剂量组与对照组相比,差异有统计学意义(P<0.05)。结论发育期接触磺胺二甲嘧啶,可以通过干扰体内甲状腺素水平从而影响大鼠身体及智力的发育。

Objective To explore the effects of sulphamethazine on the early development of SD rats. Methods Dams were given sulphamethazine 0, 50, 100 and 200 mg/(kg ·d) respectively from gestational day (GD) 7 to postpartum day (P) 21. Each offspring were weighted twice per week; the time of eye-opening were recorded. Trunk blood was collected for measurement of serum free T4 and TSH on every 5 day after birth. The function of learning and remembering of offspring was measured by water maze experiment. Results The body weight and serum free T4 level of offspring exposed to 100, 200 mg/kg sulphamethazine were significantly reduced compared with those of controls (P<0.05). The delay of eye-openning and the deficiency on learning and remembering, which is dose-related, were observed in offspring exposed to sulphamethazine. Conclusion Developmental exposure to sulphamethazine might affect the development of body and intelligence by disrupting thyroxine.