摘要
以小麦与多枝赖草属间杂种花药培养获得的纯合后代纯系为材料,经过细胞学观察、田间选拔、抗性鉴定、综合农艺性状调查以及小区产量对比试验,从中初步筛选鉴定出具有外源耐盐性状、且农艺性状好的材料,再经分子标记(GISH)鉴定纯合易位系。表明花药培养可有效克服小麦远缘杂交后代的疯狂分离,并可促进染色体的结构变异,在短时间内获得大量具有丰富变异且可稳定遗传的后代,大大缩短了小麦远缘杂交导入外源基因的年限。
Pure lines from anther culture of intergeneric hybrids between wheat and Leymus multicaulis were used to develop pure translocation lines with salt-tolerance and ideal agronomic characteristics through cytological detection, field selection,resistance assessment,comprehensive observation of agricultural characteristics,yield trials,and molecular markers (GISH).Results showed that anther culture can obtain large number of breed-true variants in a short time by avoiding excessive segregation of posterity and promoting structural variation of chromosomes in offspring from wheat distant hybridization.This greatly shortened the time in introduction of alien genes to wheat through distant hybridization.
出处
《华北农学报》
CSCD
北大核心
2004年第1期40-43,共4页
Acta Agriculturae Boreali-Sinica
基金
河北省自然科学基金项目(301443)
关键词
小麦
多枝赖草
耐盐纯合易位系
培育技术
GISH鉴定
Wheat
Leymus muticaulis
Intergeneric hybrids
Anther culture
Translocation lines
GISH
