甜菜细胞质雄性不育系及保持系叶绿体DNA的RAPD分析

RAPD Analysis of Chloroplast DNA of Cytoplasmic Male Sterile Line and It′s Maintainer Line in Sugarbeet

对细胞质雄性不育甜菜的不育系及其保持系的叶绿体DNA(ctDNA)进行了RAPD分析,结果表明:在所选的70个引物中,甜菜细胞质雄性不育系和保持系叶绿体DNA之间不存在差异性。与此同时,对叶绿体DNA的提取方法进行了改进,在不需密度梯度离心条件下即可获得高纯度的DNA;优化了甜菜叶绿体的RAPD的反应条件。

Chloroplast DNA(ctDNA) come from cytoplasmic male sterile(CMS) line and its maintainer line of sugarbeet was studied by means of random amplified polymorphic DNAs(RAPD).The fingerprints of the amplification by polymerase chain reaction (PCR) of random DNA segments was analysised .Using 2 parts variaties of cytoplastic sterile male sugarbeet in conjunction with 70 primers show polymorphic. The results indicate :in the 70 primers by selected,there is no difference between CMS line and its maintainer line in ctDNA. Meanwhile we have improved the extraction method of ctDNA ,also optimized the program of RAPD.