Ca^(2+)对苹果果实过氧化物酶活性及其分泌的影响

The Effect of Ca^(2+) on Peroxidase Activity and Secretion of Apple Fruit Tissues

以苹果组织为试材,试验结果表明,100mmol/L的高浓度Ca2+能明显提高苹果果实POD活性,促进其分泌,低浓度Ca2+的效果不显著;5mmol/L的EGTA促进POD分泌,对POD活性的影响不显著;10mmol/L的LaCl3明显降低POD活性,促进其分泌;0.1mmol/L的Verapamil、0.1mmol/L的CPZ和0.1mmol/L的TFP提高POD活性,但抑制POD分泌。这些说明苹果果实POD活性及其分泌可能受细胞内的Ca2+和CaM所调控。

The results of the application with apple tissue showed that higher concentration Ca^(2+)(100 (mmol/L)) enhanced peroxidase(POD)activity and stimulated POD secretion significantly,but the effect of low concentration Ca^(2+) was no marked.EGTA (5 (mmol/L)) accelerated the POD secretion,but had no obvious effect on POD activity.La^(3+) (10 (mmol/L))decreased POD activity,but promoted POD secretion significantly,respectively.Verapamil(0.1 (mmol/L)),CPZ(0.1 (mmol/L)) and TFP(0.1 (mmol/L))increased the POD activity,but inhibited POD secretion.Thus it is suggested that the POD activity and secretion of apple fruit tissues may be regulated by intercellular Ca^(2+)-CaM.