产L-乳酸米根霉PW352特性及低能离子注入诱变高产菌株研究

Characteristics of L-lactic acid producing strain Rhizopus oryzae PW352 and high productive mutant by low energy ions implantation

为了获得更适于工业生产的产乳酸菌株 ,以葡萄糖为碳源对PW35 2菌株特性进行了研究 ,并在此基础上采用离子束诱变方法 ,对米根霉PW 35 2进行改良 ,获得高产L(+) 乳酸菌株RE330 3,产酸能力达 131～ 136 g L ,最高可达 14 0g L ,糖转化率为 86 %～ 90 % ,产酸比PW 35 2提高 75 %。

In order to obtain higher L-lactic acid yield industrial strain, the characteristics and fermentation conditions of Rhizopus oryzae PW352 using glucose as carbon source were studied. The original strain PW352 was mutated by means of N + ions implantation and a mutant strain RE3303 was obtained. Its lactic acid production ability was increased by 75% than that of the original. The concentration of L-lactic acid reached to 131～136g/L and the conversion rate of glucose was as high as 86%～90% under the condition of 150g/L glucose in fermentation medium.