多元混菌发酵对纤维素酶活性的影响

Effects of mixed poly-fermentation on cellulase activity

研究了两种曲霉 (UF2和UA8)二元混菌体系和两种曲霉与 1种酵母菌组成的三元混菌体系混合发酵对纤维素酶系三种酶组分活性的影响。结果表明 :两种霉菌按一定比例接种进行混合发酵时三种纤维素酶组分的活性较单菌发酵大幅度提高 ,滤纸酶 (FPA)、微晶纤维素酶 (AVI)和羧甲基纤维素酶 (CMC)活性分别较UA8单菌发酵提高 2 .2 %～ 5 1.1%、2 0 .7%～ 332 .6 %和2 9.4 %～ 2 99.6 % ;向由两种霉菌组成的二元混菌发酵体系中接入酵母菌可显著降低 3种纤维素酶组分的活性 ;三菌混合发酵能使纤维素酶 3组分的产酶高峰出现时间较双菌混合发酵滞后约 2 4h ,但三菌与双菌混合发酵 3种纤维素酶组分的酶活峰值无明显差异 ;

The effects of the composition on cellulase activities were studied by mixed fermentation. One system was composed of Aspergillus niger and Aspergillus fumigatus, the other consisted of Aspergilluses and Candida The results showed that cellulase activities were greatly increased in comparison with the fermentation of the dual Aspergilluses inoculated in a given proportion for fermentation. Compared with sole fermentation of UA8, the activities of FPA, avicelase and cmcase were increased from 2.2% to 51.1%, from 20.7% to 332.6% and from 29.6% to 299.6% respectively. The activity of cellulase system was significantly reduced after Candida was inoculated in the mixed fermentation of the dual Aspergilluses. The peak values of enzyme production fermentation in the mixture of triple microorganisms showed a lag about 24 hours to compare with that of the dual microbes. However the difference in the peak values of cellulase was not obvious in the dual systems. The mixed fermentation of two dual microbes may be advantageous to shorten the fermentation cycle in cellulase production.