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稻米中的普鲁兰酶与不溶性直链淀粉的关系 被引量:7

RELATIONSHIP BETEEN RICE PULLULANASE AND INSOLUBLE AMYLOSE
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摘要 本文采用人工模拟稻谷储藏条件,证实了稻米在37℃和4℃交替处理的情况下,总的普鲁兰酶活力不变、活性酶活力减小、抑制酶活力增多;全部直链淀粉含量无明显变化,不溶性直链淀粉含量逐渐增多。不溶性直链淀粉的含量与普鲁兰酶活力成负相关;不溶性直链淀粉的含量与直链淀粉和支链淀粉的比值成正相关,其中四个样品为显著水平,一个样品接近显著水平。由此推测,不溶性直链淀粉的来源是由于稻米在代谢过程中,细胞内的普鲁兰酶作用于直链淀粉和支链淀粉的α—1.6葡萄糖苷键而产生。 The artificially initated condition of rice storage under certain treatment at 37℃ and 4℃ proved that the total Pullulanase content was unchangeable, the active enzyme content was decreasing and the inactive one was increasing, with no apparent changes of the total amylose content but with and increase of insoluble amylose. There was a negative correlation between isoluble amylose content and the activity of active enzyme but a positive correlation between the insoluble amylose content and the ratio of amylose content to amylopectin. Four of the samples were on significant levels and the other close to the significant. All this showd that the insoluble amylose was obtained from α-1. 6-glucoside lindage in amylose and amylopectin hydrolyzed by Pullulanase in rice during rice metabolisem.
出处 《郑州粮食学院学报》 1992年第4期1-9,共9页 Journal of Zhengzhou Institute of Technology
基金 国家自然科学基金
关键词 不溶性 糖淀粉 普鲁兰酶 稻米 insoluble amylose pullulanase active enzyme ratio of amylose content to amylopectin rice aging
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参考文献5

  • 1程池,张锡清.普鲁兰酶活性测定方法[J]食品与发酵工业,1988(06).
  • 2张名光.大米酶活力的研究[J]粮食贮藏,1981(04).
  • 3刘瑞征.关于稻米陈化中蒸煮品质变化机理的不同见解[J]粮食贮藏,1980(01).
  • 4(英)伯奇(Birch,G.G.)等主编,郑寿亭等.酶与食品加工[M]轻工业出版社,1991.
  • 5北京大学生物系生物化学教研室.生物化学实验指导[M]人民教育出版社,1979.

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