摘要
目的建立生发颗粒中大黄素含量的测定方法。方法采用高效液相色谱法,色谱柱:YMC-PackODS-AC18柱(250×4.6mm,5μm),甲醇-0.25%磷酸液(80∶20)为流动相,检测波长254nm。结果大黄素的线性范围是0.021μg～0.155μg,r=0.9999;平均回收率为99.03%,RSD=1.40%(n=6)。结论该法可用于测定生发颗粒中大黄素的含量。
Objective To establish a method for determination of emodin content in Shengfa granule.Methods HPLC was used.Mobile phase consisted of methanol-0.25 %phosphoric acid (80 ∶20) with UV detection at 254 nm,the analytical column was YMC-Pack ODS-A.Results The linear range of emodin was 0.021 μg～0.155 μg(r=0.9999)while the average recovery rate was 99.03 %(RSD=1.40 %,n=6).Conclusion The methods is suitable for determination of emodin content in Shengfa granule.
出处
《中药新药与临床药理》
CAS
CSCD
2004年第2期114-116,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
