薄层扫描法测定牵正膏中桂皮醛的含量

Determination of Cinnamaldehyde Content in Qianzheng Plaster by Thin Layer Chromatography Scanning Method

目的建立牵正膏中桂皮醛含量的薄层扫描测定方法。方法以蒸馏法提取牵正膏中的桂皮醛,以薄层扫描法测定其含量。硅胶G薄层板,展开剂为石油醚(60～90℃)-乙酸乙酯(17∶3),扫描波长269nm。结果桂皮醛在0.81μg～4.05μg点样量范围内线性关系良好(r=0.9993),平均回收率为95.23%,RSD=1.39%。结论该方法准确,灵敏,重现性好。

Objective To establish a thin layer chromatography scanning method for the determination of cinnamaldehyde content in Qianzheng Plaster.Method Cinnamaldehyde was extracted from Qianzheng Plaster by distillation method.Silica G was used as the thin-layer plate;petroleum ether (60～90℃) and acetic ethyl acetate (17 ∶3)served as the mobile phase and the scanning wavelength was 269 nm.Results A good linearity was obtained in the range of 0.81μg～4.05 μg (r=0.9993).The mean recovery rate was 95.23 %and RSD=1.39 %.Conclusion This method is accurate and sensitive with good reproducibility.