摘要
GP41蛋白二级结构预测显示 ,前 1 /2片段的 N端 ( 4～ 2 6位氨基酸 )和 C端 ( 1 67～ 1 89位氨基酸 )各有一个富含疏水氨基酸的穿膜螺旋 (可能性 >0 .9) ,分别从 N1 (前 1 /2片段无 C端穿膜螺旋 )的 N端和 N6(前1 /2片段无 N端穿膜螺旋 )的 C端进行缺失构建一系列缺失突变体 ,只有 p ET-N2 ,p ET-N3 ,p ET-N4,p ET-N5在大肠杆菌中获得高效表达 ,其表达量占菌体总蛋白的 2 0 % ,Western blot显示表达蛋白与 HIV阳性血清有良好的反应性 .而 p ET-N1 ,p ET-N6在大肠杆菌中表达量很低或不表达 .从而证明 1～ 6位 ,1 84～ 2 0 1位氨基酸是影响 gp41基因 N端 1
Prediction of protein secondary structure of HIV GP41 showed that there are two transmembrane helixes with abundance hydrophobic amino acids in both N and C of former 1/2 segment of HIV GP41(probability>0.9). we researched expression of truncated N1(Aa l 175) and N6(Aa 24～201) segments from former 1/2 segment of gp 41 gene in the PET expression system to illustrate effects of specific sequence of gp 41 on the protein expression. The segments N2,N3,N4,N5 can be over producted, and the products take about 20% in the total bacteria body protein. In the Western blot test, the protein product can react with HIV positive serum. The segments N1 and N6 can be expressed a little or even not. Our experiment slowed that amino acids 1～6 and 184～201 are the key factor that affects the expression of N 1/2 segment from gp 41 gene.
出处
《南开大学学报（自然科学版）》
CAS
CSCD
北大核心
2004年第1期64-68,共5页
Acta Scientiarum Naturalium Universitatis Nankaiensis
基金
高等学校博士学科点专项科研基金资助项目( 2 0 0 0 0 0 5 5 1 2 )
