酶法生产L-半胱氨酸培养基的响应面分析优化

THE OPTIMUM OF ENZYME CONVERSION DL-2-AMINO-Δ~2-THIAZOLINE-4-CARBOXYLIC ACID TO L-CYSTEINE MEDIUM BASED ON RESPONSE SURFACE ANALYSIS

利用 SAS软件的 Plackett-Burman设计法对微生物酶法生产 L-半胱氨酸的培养基组成进行筛选 ,筛选出 4个影响较大的重要因素 ,即葡萄糖、玉米浆、尿素、DL-2 -氨基 -Δ2 -噻唑啉 -4-羧酸 ( DL-2 -mino-Δ2 -Thiazo-line-4-Carboxylic Acid,缩写为 DL-ATC) ,并且用 SAS软件二次响应面分析法进行回归分析 ,得到了各因素的最佳水平值 ,经五批培养验证 ,预测值与验证试验平均值接近 .5 L发酵罐培养试验表明 ,在优化条件细胞酶活力提高 2 5 .4% .

Two level factorials designs of Plackett Burman were constructed to select culture medium components of L cysteine.. Four important components which are glucose, corn liquid, urea, DL 2 Amino Δ 2 Thiazoline 4 Carboxylic Acid (DL ATC) were screened. The optimized level of these factors were determined by response surface analysis. After five batches cultivation, predominant value was consistent with mean value of verification test. 5L fenmenter cultivatrion showed that cell enzyme activity increased 25.4% at optimized condition.